Composite

Part:BBa_K2495001:Design

Designed by: Adrienne Chang   Group: iGEM17_NYU_Abu_Dhabi   (2017-10-09)


Device expressing the putative O-antigen transporter under control of an arabinose inducible promote


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1225
    Illegal NheI site found at 1248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2108
    Illegal SapI site found at 961
    Illegal SapI site found at 1443


Design Notes

We added an arabinose promoter because future expression of the O-antigen transporter may be useful for further experimentation.


Source

One of the twelve proteins needed to express the O-antigen transporter is coded by the rfbE gene which is found in E.coli O157:H7 strain and confers protection against Shiga-toxin infection.

References